TABLE OF CONTENTS

 

Procedures common to the maintenance of Schistosoma mansoni, S. haematobium, and S. japonicum

Preparation of suitable water for snails

Changing containers of snails

Gel snail food preparation

Exposure of snails to miracidia

Preparation of mud base medium for growth of Nostoc

Preparation of soluble egg antigen (SEA)

 

 

Procedures common to the maintenance of S. mansoni and S. haematobium, and their intermediate hosts

Egg clutch collection

Growth of immature Biomphalaria glabrata

Growth conditions until patency

Microscopic evaluation of sporocyst development in live Biomphalaria glabrata

Fixing Biomphalaria glabrata snails for dissection

Shedding snails

Behavior of Schistosoma mansoni cercariae

Counting cercariae

Preparation of in vitro schistosomules

Culturing schistosomula

A histochemical method for identifying sex of Schistosoma mansoni cercariae

Molecular technique for sexing cercariae

Collection of lung Schistosoma mansoni schistosomules

Perfusion of adult worms from mice

Control of rotifers and other commensals on/within Biomphalaria glabrata snails

Tail exposure method for Schistosoma mansoni cercarial exposure

Anesthesia techniques for mice (for abdominal cercarial exposure)

Collection of eggs from tissues

Maintenance of Bulinus truncatus truncatus snails

Exposure of hamsters to Schistosoma haematobium cercariae

Perfusion of Schistosoma haematobium from hamsters

Collection of Schistosoma haematobium eggs from hamsters

 

 

Procedures specific for the maintenance of S. japonicum in their Oncomelania spp. snail hosts

Care of breeding pairs of Oncomelania snails

Collecting, isolating, and hatching Oncomelania eggs

Exposing Oncomelania snails to Schistosoma japonicum miracidia

Schistosoma japonicum cercarial collection and exposure of mice

Collecting Schistosoma japonicum eggs from mouse tissues