NIAID Schistosomiasis Resource Center

Collecting Schistosoma japonicum eggs from mouse tissues

Authors Yung-san Liang and Mei-Shei Su

Introduction

The procedure for collection of S. japonicum eggs from mouse tissues is essentially the same as that used for collecting S. mansoni eggs from mouse tissues. Recoverable S. japonicum eggs will be found in the liver and in the intestinal walls.

Equipment

Spray apparatus (2-gallon pump-type, typically found in hardware stores)

Stainless steel sieves of decreasing pore sizes (Newark Wire Cloth; mesh openings of 420 µm,

180 µm, 105 µm, and 45 µm) (Figure)

Dissecting instruments

Waring blender with variable speed control

Glass petri dishes (100 mm diameter) with flat bottoms

Falcon #2340 cell strainer, 40 µm nylon (Figure)

Pasteur pipettes

Light box

Materials and reagents

1.2% NaCl (2 liters)

S. japonicum-infected mice

Procedure

See SOP for collection of S. mansoni eggs from tissues

Follow-up comments/recommendations

S. japonicum eggs are smaller than those of S. mansoni. As a consequence, 1 ml of the settled egg pellet will contain approximately 1,000,000 eggs.

References

Lewis, F.A. 1999. Schistosomiasis, in Current Protocols in Immunology, John Wiley and Sons, Inc., (R. Coico, Ed) Vol III, 19.1.1. – 19.1.28.

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		*Collecting Schistosoma japonicum* eggs from mouse tissues Authors Yung-san Liang and Mei-Shei Su Introduction** The procedure for collection of S. japonicum eggs from mouse tissues is essentially the same as that used for collecting S. mansoni eggs from mouse tissues. Recoverable S. japonicum eggs will be found in the liver and in the intestinal walls. Equipment Spray apparatus (2-gallon pump-type, typically found in hardware stores) Stainless steel sieves of decreasing pore sizes (Newark Wire Cloth; mesh openings of 420 µm, 180 µm, 105 µm, and 45 µm) (Figure) Dissecting instruments Waring blender with variable speed control Glass petri dishes (100 mm diameter) with flat bottoms Falcon #2340 cell strainer, 40 µm nylon (Figure) Pasteur pipettes Light box Materials and reagents 1.2% NaCl (2 liters) S. japonicum-infected mice Procedure See SOP for collection of S. mansoni eggs from tissues Follow-up comments/recommendations S. japonicum eggs are smaller than those of S. mansoni. As a consequence, 1 ml of the settled egg pellet will contain approximately 1,000,000 eggs. References Lewis, F.A. 1999. Schistosomiasis, in Current Protocols in Immunology, John Wiley and Sons, Inc., (R. Coico, Ed) Vol III, 19.1.1. – 19.1.28.